Human TLR2 Stable Cell Line

Name	Human TLR2 Stable Cell Line
Supplier	Novus Biologicals
Catalog	NBP2-26266
Category	Cell Line
Prices	$849.00
Sizes	1 vial
Gene	TLR2
Species	Human
Growth Mode	Adherent Morphology: Epithelial
Description	The TLR2 stable cell line can be used for TLR2 flow cytometric calibration and detection control as well as TLR2-dependent functional assays. TLR2 expression in these cells has been validated by Western blotting (fig. 1) and flow cytometry (fig. 2). Functional activity of this cell line has been validated using the NF-kB/SEAPorter™ Assay Kit (NBP2-25286, fig. 3).
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Product images

Western Blot: Human TLR2 Stable Cell Line [NBP2-26266] - TLR2 expression in the TLR2 stable cell line using an HA antibody (20 ug total protein/lane). Legend. Vect: Vector/HEK 293 TLR2: TLR2 stable cell line.

Flow Cytometry: Human TLR2 Stable Cell Line [NBP2-26266] - Surface expression of TLR2 in the TLR2 stable cell line was analyzed by flow cytometry using a PE-conjugated TLR2 antibody and compared with the Vector/HEK 293 control. Surface TLR Staining Flow Kit was used for this test.

Functional: Human TLR2 Stable Cell Line [NBP2-26266] - Analysis of the TLR2 stable cell line. The assay was performed using the NF-kB SEAPorter Assay Kit. The Vector/HEK 293 control and TLR2 stable cell line were transfected with NF-kB/SEAP reporter plasmid for 16 h. Cells were stimulated with Pam3CSK4 or MALP-2 for 24 h followed by SEAP assay. Note: The TLR2 stable cell line can be activated by either Pam3CSK4 or MALP-2 since endogenous levels of TLR2 and TLR6 are present in HEK 293 cells.